S3: Alternative testing methods for nanotoxicology

Alternative testing methods for nanotoxicology

S3: Alternative testing methods for nanotoxicology, OpenTox Asia 2017

Rawiwan Maniratanachote


National Nanotechnology Center (NANOTEC)


Senior Research Scientist


Co-authors: Sasitorn Aueviriyavit, Chitrada Kaweeteerawat, and Wittaya Pimtong

Affiliation: National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Pathum Thani 12120, Thailand.


Nanotechnology is a rapidly growing area of research and development in various industrial sectors. The utilization of nanomaterials can be ubiquitously found in products including electronics, household items, medical devices, food and cosmetics. Thus, public safety of different nanomaterials is an important concern. Reliable toxicological testing models and methods are required to gain insightful and thorough understanding of how the particles interact and potentially cause adverse effects to human health and the environment. We are currently in the stage of moving forward in the area of humane science to reduce, refine and replacement of animals, known as the 3Rs principle, in the research and development of consumer products. Meanwhile, the use of animals for toxicity testing has begun to fade out as alternative methods are increasingly developed and validated, which are also useful in the field of nanotoxicology. This talk will highlight in vitro models of cells in hematological system, human tissues, as well as microorganisms for investigating effects of metal-based nanomaterials on human health and the environment. In addition, zebrafish embryo is now of increasing interests as an alternative model for scientific purposes. This model allows for studying of embryonic development and various biomolecular endpoint.