Poster: Nanoparticles in inverted 3D systems

Evaluation of in vitro toxicity of nanoparticles in inverted 3D culture systems

Soojin Kim, Jung-Hwa Oh, Se-Myo Park, Mi-Sun Choi, A-young Choi, Eun-Hee Lee, Heeyoung Yang and Seokjoo Yoon, Molecular Toxicology Research Center, Department of Predictive Toxicology, Korea Institute of Toxicology


Seokjoo Yoon, Korea Institute of Toxicology

OpenTox Euro 2014 Poster: Nanoparticles in inverted 3D culture systems

To assess the potential toxicity of manufactured or engineered nanoparticles, traditional in vitro toxicity studies have been performed using normal 2D culture system.

Different outcomes of toxicity have been argued in vitro studies according to their own physicochemical properties in contrary to small chemicals. Here, we introduce the inverted 3D culture systems based on 96-well plates to examine the cytotoxic effects of nanoparticles.

Silica nanoparticles with four different sizes (70, 150, 150, and 300 nm) and silver nanoparticles are exposed to 3D hydrogel droplets containing HepG2 cells on the top insert pillar. The cytotoxic effects of nanoparticles are evaluated using tetrazolium reduction and ATP assay and then compared to the results from normal 2D culture exposure. The agglomeration and total numbers of silica and silver nanoparticles depending on volumes provoke the artifacts of cytotoxicity in normal 2D culture system.

The results reveal that 3D inverted culture system shows the more accurate cytotoxic effects comparing to normal 2D culture, by reflecting nanoparticles would be exposed to more actual transporting dose in our system. This system would significantly improve the predictive power of in vitro nanotoxicity assessment.