Poster: Toxicological Biomarkers In Multi-Organs

Evaluation Of Toxicological Biomarkers In Multi-Organs Including Liver, Kidney And Aorta Of Rats Exposed To Thioacetamide

Yoonjin Kim, Ji-Youn Lim and Donggeun Sul, Graduate School of Medicine, Korea University, Kyoung-Sik Moon and Yong-Bum Kim, Department of Toxicological Evaluation and Research

OpenTox Euro 2014 Poster: Toxicological Biomarkers In Multi-Organs

Proteomic analysis was conducted to determine the effects of thioacetamide on multi-organs including liver, kidney and aorta of rats.

Rats were exposed to three different concentrations of thioacetamide (0, 10, 30 mg/kg bw) for 4 weeks in oral administration. AST, ALT, TBIL, and ALP increased significantly in the exposed group when compared with control group. Large size two dimensional electrophoresis using three different pI ranges (3-5, 4-7, 6-9) showed the presence of 3325, 3267, and 2801 in livers, kidney and aorta, respectively.

Thirty nine (32 up-and 7 down-regulated), thirty one (4 up-and 27 down-regulated) and twenty four (18 up- and 6 down-regulated) proteins were identified as toxicological biomarkers in liver, kidney and aorta, respectively. Proteins involved in apoptosis, immune response, energy metabolism, cell cycle, signaling, transportation, and cell structure were found to be up- and down-regulated. Among these, three proteins were found in all organs and five proteins were found in liver and kidney. Only one protein was found in liver and aorta. Of these 94 proteins, 23 proteins were confirmed by western blot assay.

They could used as potential toxicological biomarkers of thioacetamide.