Session 5: The ChemScreen project
BioDetection Systems BV, Netherlands
ABSTRACT CONTENT / DETAILS:
There is a great need for rapid testing strategies for reproductive toxicity testing, avoiding animal use. The EU Framework program 7 project ChemScreen aimed to fill this gap in a pragmatic manner preferably using validated existing tools and place them in an innovative alternative testing strategy.
In our approach we combined knowledge on critical processes affected by reproductive toxicants with knowledge on the mechanistic basis of such effects. We used in silico methods for prescreening chemicals for relevant toxic effects aiming at reduced testing needs. For those chemicals that need testing we have set up an in vitro screening panel that includes mechanistic high throughput methods and lower throughput assays with apical endpoints.
Developed modules for rapid exposure predictions via diverse exposure routes greatly improved predictivity of the in vitro tests. As a further step, we have generated examples how to predict reproductive toxicity of chemicals using available data.
We are actively engaged in promoting regulatory acceptance of the tools developed as an essential step towards practical application. With this, a significant saving in animal use and associated costs seems very feasible.