Curcumin-loaded hayaluronic acid nanohydrogels as promising scaffolds for odontogenic differentiation of dental pulp stem cells
Introduction. Periodontitis can result in progressive damage of periodontal tissues which support the tooth. Developing novel biomaterials in dental regeneration has markedly enhanced the current treatment opportunities. The present investigation evaluated the curcumin loaded hyaluronic acid hydrogels as promising scaffolds for odontogenic tissue engineering.
Methods. The scaffolds were prepared through anti solvent method, and then the physicochemical specification and the cytotoxic effects and differentiation of dental pulp stem cells were determined.
Results. The nano-scaffolds were successfully synthetized with a mono-dispersed nano scale diameter (290.10 nm), negative surface charge and uniform network-shaped morphology. Human dental pulp stem cells were harvested on the developed nanoscafolds. Nanoscafolds exhibited a significant proliferative effect and induced the differentiation of the stem cells.
Conclusion. The incorporation of different compounds to nano-scaffolds such as curcumin not only increases the physiochemical properties of scaffolds in stem cell proliferation and differentiation but also might exert anti-bacterial and anti-inflammatory effects.